Antibacterial Potential of Green Synthesize Gold Nanoparticles Using Pomegranate Peel Extract on MDR Uropathogenic Bacteria

Abstract

Urinary tract infections have become a public health concern in recent
years due to antibiotic resistance. The purpose of this study is to assess
the antibacterial activity of green synthesize gold nanoparticles on
MDR bacteria. One hundred urine samples were collected from patients
with urinary tract infections, the identification and antibiotic
susceptibility tests of bacterial isolates were achieved by using the
Vitek 2 compact system. The green AuNPs was synthesized by using
extract of pomegranate peel, then detected visually by changing in
color, and characterized by using Ultra-Violet/ Visible spectroscopy
and SEM. The antibacterial activity of AuNPs is determined by agar
well diffusing. Out of 100 urine samples, seventy-three bacterial
isolates were 38 (52%) Gram-negative bacteria and 35 (47.9%) were
Gram-positive bacteria. Among Gram-negative bacteria, E. Coli 26
(68.42%) was the most common species. While the most common
Gram-positive bacteria Streptococcus epidermidis 15 (42.8%). Gold
Nanoparticles were detected visually by changing in color to violet and
characterized using Ultra-Violet/ Visible spectroscopy (UVVis) that
showed absorbance peak (550 nm). Scanning Electron Microscopy
(SEM) analyzer showed the size ranged from (64-76nm) with spherical
shape. The gold nanoparticles have the ability to inhibit the growth of
E. Coli (13mm) and Staphylococcus epidermidis (12mm) bacteria.
Green gold nanoparticles can be used eco-friendlily (more safety, less
toxicity) as an alternative to antibiotics against antibiotic resistance
bacteria.